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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Anesthesiology. 2012 Oct;117(4):836–846. doi: 10.1097/ALN.0b013e3182655e73

Figure 6. Post-ischemic treatment of intralipid and cyclosporine-A decreases cardiac reactive oxygen species generation as well as mitochondrial superoxide production.

Figure 6

A. Representative dihidroethidium staining of transverse heart sections subjected to 20min of global ischemia followed by 5min reperfusion with phosphate buffered saline (control group, CTRL) (black), 1% intralipidgroup(ILP) (red) and 1.5 μM cyclosporine-A group(CsA)(blue). B. Fluorescence quantification of dihydroethidium (DHE) staining: average intensity represents area×fluorescence intensity and is normalized to CTRL (**p<0.001 vs. CTRL, n=3). C. Superoxide production in isolated mitochondria using electron spin resonance in CTRL (black), 1% intralipid (red) and 1.5 μMcyclosporine-A group(blue). **p=0.005 ILP vs. CTRL; *p=0.041 CsA vs. CTRL (n=4).