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. Author manuscript; available in PMC: 2013 Sep 10.
Published in final edited form as: Dev Biol. 2009 Jun 22;333(1):26–36. doi: 10.1016/j.ydbio.2009.06.019

Figure 8. Dazap2 activity is Cdx4 independent.

Figure 8

mRNAs for dazap2 (250pg, B), cdx-EnR (250pg, C) or a combination of dazap2 and cdx-EnR (250pg each, D) were injected into one cell of 4-cell stage embryos (all were co-injected with lacZ mRNA). Stage 18 embryos are shown in dorsal views, with anterior at the top. Dazap2 injections induce ectopic hoxb9 expression (B, arrowhead) while cdx4-EnR overexpression blocks hoxb9 induction (C, arrow). Combined Dazap2 and cdx-EnR injection results in robust hoxb9 induction (D, arrowhead). Parallel experiments in explants (E) show that Dazap2 induces hoxb9 either alone (lane 4) or in the presence of Cdx-EnR (lane 5).