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. Author manuscript; available in PMC: 2013 Sep 10.
Published in final edited form as: Dev Biol. 2009 Jun 22;333(1):26–36. doi: 10.1016/j.ydbio.2009.06.019

Figure 9. Dazap2 activity does not depend on canonical WNT signaling.

Figure 9

(A) RT-PCR was performed using whole embryos injected with fgf8a (100pg/cell) either alone or in combination with β-catenin MO (either 40ng/cell or 60ng/cell) into two cells at the 2-cell stage, animal caps were dissected and then aged until stage 19 (WE, whole embryo sibling controls; -RT, no reverse transcriptase control; Ni, non-injected explant control). Hoxb9 expression is lost at both concentration of β-cat MO (lane 5,6) when compared to Fgf8a alone (lane 4). (B) When the experiment is repeated using Dazap2 (125pg/cell), β-catenin MO is unable to block Dazap2 induction of HoxB9 expression (compare lane 4, with co-injection lane 5 and 6).