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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Prev Res (Phila). 2013 Jul 12;6(9):949–958. doi: 10.1158/1940-6207.CAPR-13-0097

Fig. 5. Induction of hydrogen peroxide by Chel A in Cl41 cells.

Fig. 5

(A) diagram of ROS generation and decomposition. (B and C) Cl41 cells were pretreated with 2′,7′-dichlorfluorescein-diacetate (DCFH) (B) or dihydroethidium (HE) (C), respectively, for 0.5 h, followed by being treated with Chel A for indicated time periods. Then cells were washed with PBS, and the fluorescence intensity was detected. The results were shown as the induction of each time point relative to 0 min. The symbol (*) indicates a significant increase as compared with that of 0 min (p<0.05). Each bar indicates the mean and standard deviation from three independent experiments.