. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Mol Phylogenet Evol. 2013 May 10;69(1):95–108. doi: 10.1016/j.ympev.2013.04.032

Table 2.

Details of loci used in this study. Tm is the annealing temperature. More than one temperature is listed for primers that were used in touchdown PCRs.

Gene	Primer	Tm	# Ind	# Sbgn	Chars(PIC)^d
nuclear

n(l)tid ^a	ntidL-GGGYCGCATCTTTGAGCACAAATGG	60C	26	9	618(71)
n(l)tid ^a	ntidR-TGCTGGGATAGGTGTTCCARCARTA	60C	26	9	618(71)
cad-r ^a	Cad787F-GGSAATACGACNGCCTGYTTTGARCC	62C,58C,54C	36	12	849(208)
cad-r ^a	Cad1098R-TTNGGCAGCTGRCCNCCCAT	62C,58C,54C	36	12	849(208)
Marf ^a	MarfF1-ATGGCGGCCTAYTTGAAYCGCA	62C,58C,54C	51	12	371(84)
Marf ^a	MarfR1-AAGAAGGCGACCTTCATGTGRTC	62C,58C,54C	51	12	371(84)
gstd-1 ^b	gstd1FB-TGTGCTYTTCTAATTATAG	38C	12	7	630(76)
gstd-1 ^b	gstd1RA-GAATACWCTTTTATTWTAAG	38C	12	7	630(76)

mitochondrial

16s ^c	16sF-CCGGTTTGAACTCAGATCACGT	56C	43	12	550(25)
16s ^c	16sR-CGCCTGTTTAACAAAAACAT	56C	43	12	550(25)
co1 ^c	2183-CAACATTTATTTTGATTTTTTGG	56C	71	12	765(246)
co1 ^c	3041-TYCATTGCACTAATCTGCCATATTAG	56C	71	12	765(246)
co2 ^c	3037-ATGGCAGATTAGTGCAATGG	56C	72	13	700(214)
co2 ^c	3791-GTTTAAGAGACCAGTACTTG	56C	72	13	700(214)
nd2 ^c	192-AGCTATTGGGTTCAGACCCC	56C	35	12	559 (102)
nd2 ^c	732-GAAGTTTGGTTTAAACCTCC	56C	35	12	559 (102)

Bonacum, et al, 2001.

A. Gloss, in prep.

Simon, et al, 1994.

Maximum sequence length. Number of parsimony informative characters for ingroup taxa are in parentheses.

#Ind refers to the number of individuals sequenced for that locus for this study. #Sbgn refers to the number of subgenera that had representatives sequenced for that locus in this study.