Table 1. Calculated and experimental cAMP affinities for CAP proteins.
| CAP Protein | K 2/K 1 (ENM) | S.E.M. (n) | p Value | Mean K 2/K 1 (ITC) | S.E.M. (n) | p Value |
| Wild-type | 1.35 | 0.01 (16) | — | 1.68 | 0.04 (32) | — |
| V132A k V132/k = 0.25 | 1.59 | 0.01 (16) | <0.001 | 4.78 | 0.33 (20) | <0.001 |
| V132L k V132/k = 4 | 0.91 | 0.02 (16) | <0.001 | 0.58 | 0.03 (17) | <0.001 |
| H160L k H160/k = 0.25 | 1.05 | 0.02 (16) | <0.001 | 1.36 | 0.03 (31) | <0.001 |
| V140A k V140/k = 0.25, k C179/k = 4 | 1.02 | 0.01 (16) | <0.001 | 0.61 | 0.05 (29) | <0.001 |
| V140L, k V140/k = 4 | 1.31 | 0.01 (16) | 0.733 | 1.56 | 0.05 (27) | 0.999 |
The ratio of the second to first dissociation constants for cAMP (K 2/K 1) for wild-type and mutant CAP proteins were calculated from the ENMs or obtained by ITC. The p value is for a comparison of means to the wild-type.