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. 2013 Sep 10;11(9):e1001655. doi: 10.1371/journal.pbio.1001655

Figure 5. NtCYS interacts with and inhibits cathepsin H-like protease NtCP14.

Figure 5

(A) BiFC analysis of the interaction between NtCYS and six cathepsins in tobacco epidermal cells. Scale bars, 50 µm. (B) Proteolytic activity of recombinant NtCP14 against different substrates. The activity of NtCP14 against each substrate is expressed as the percentage of NtCP14 activity against substrate FVR-AMC. Data represent the mean ± SE of three independent experiments. (C) Co-IP of NtCYS with NtCP14-GFP. GFP was used as negative control. Immunoblotting was performed with anti-NtCYS for NtCYS and anti-GFP for NtCP14-GFP or GFP. (D) NtCYS concentration-dependent inhibition of proteolytic activity of recombinant NtCP14 towards Bz-FVR-AMC. Data represent the mean ± SE of three independent experiments. (E) Cytoplasmic localization of NtCYS-GFP and NtCP14-RFP in the onion epidermal cells. Scale bars, 50 µm.