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HeLa cells were seeded on glass coverslips and double transfected with EGFP-Rab5A Q79L and wt CLN5 or N-glycosylation mutants as indicated. The cells were chased with cycloheximide for 2 h prior to fixation. Localization of (A) CLN5 wt, N192Q, and N227Q, (B) N179Q, N252Q, N304Q, and N320Q, (C) N401, (D) N330Q in Rab5A Q79L expressing cells. Original magnification, 1,000×. Bars, 5 µm.
