Figure 4. Endo H sensitivity and deglycosylation studies of CLN5.

(A) Whole cell lysates transiently expressing N-glycosylation mutants were digested with Endo H enzyme and analyzed by Western blotting. (B) Western blotting of uncut, Endo H, or PNGase F treated whole cell lysates expressing wt CLN5, as well as whole cell lysates from CLN5 transfection in the presence of tunicamycin. Equal amount of lysates was loaded onto each well. The mouse monoclonal anti-Myc antibody was used to detect CLN5.