Figure 5. Stability of N-glycosylation deficient CLN5 proteins.

HeLa cells were transfected with wt CLN5 or N-glycosylation mutants as indicated for 20 h after which cycloheximide chase was performed for 0, 2, and 4 h. Cell pellets from 0, 2, and 4 h and medium samples from 2 and 4 h are shown. No samples were loaded in lanes labeled (−). The mouse monoclonal anti-Myc antibody was used to detect CLN5.