. 2013 Sep 10;8(9):e74436. doi: 10.1371/journal.pone.0074436

Table 1. Inhibition of the mitochondrial cytochrome c oxidase activity by Ca²⁺.

	Inhibition, %%
	Heart	Skeletal muscle	Liver	Kidney

Rat mitochondria	50±5	60±5	80±2	76±4
Bovine mitochondria	57±4	–	–	–

Oxidation of 15 µM ferrocytochrome c by mitochondria from different tissues (0.5–1.5 mg protein/ml) was followed spectrophotometrically under low-turnover conditions as described in “Materials and Methods” and legend to Figure 7 . The data correspond to inhibition induced by addition of excess Ca²⁺ (200 µM on the backgrpound of 100 µM EGTA). The data are average ± SE for 3–5 measurements.

Table 1. Inhibition of the mitochondrial cytochrome c oxidase activity by Ca2+.

Table 1. Inhibition of the mitochondrial cytochrome c oxidase activity by Ca²⁺.