Figure 4. Co-immunoprecipitation (Co-IP) from biotinylated H. pylori extract and recombinant proteins co-expressed in E. coli showing interacting partners of CagI.

(A) Western blots showing co-immunoprecipitation of biotinylated proteins by anti-CagI antibody from biotin labeled H. pylori extract. Blots were probed with HRP-conjugated anti-biotin antibody (α-biotin) and anti-CagI antibody (α-CagI). Arrows indicate biotinylated protein bands. (B and C) Western blots showing Co-IP of recombinant Hp1489 (GST-tag) and CagI (without tag) by anti-CagI and anti-Hp1489 antibodies respectively. Antibodies used in Western blots are indicated. M-indicates standard protein size markers.