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(A) Equal amounts (OD600-2.0) of all indicated Hp cells were lysed in SDS sample buffer and were separated in SDS-PAGE, transferred to PVDF membrane and Western blotted. (B) Stabilities of Cag-T4SS components CagX, CagT, CagM, CagF, and CagZ in isogenic HpΔcagI mutant strain. Antibodies used are indicated. OMP was used as loading control.
