Table 1. Oligonucleotides used in the present study.
fΔ32NcagI NcoI | 5’-CATGCCATGGGAGCCATTCAGGCGGACGCAC-3’ |
---|---|
rcagI HindIII | 5’-CCCAAGCTTTCATTTGACAATAACTTTAGA-3’ |
fcagH BamH1 | 5’-CCGGATCCCCGCAAATGACCGCTATCATG-3’ |
fHp1489BamHI | 5’-CCGGATCCGTTGATGGGATTTCTAAAAC-3’ |
rHp1489Sal1 | 5’-ATGCGTCGACTTAATAAACAAATTCATAAAATAA-3’ |
rcagH Sal1 | 5’-ATGCGTCGACTCACTTCACGATTATTTTAGTTTG-3’ |
fcatXhoI | 5’-CCGCTCGAGTTGTTGATGGGGCAGGCA-3’ |
rcatBamHI | 5’-GCGGATCCTTATTTATCTCTGACAAGAG-3’ |
fcagIBamHI | 5’-GGGGATCCGTCGACTCTAAAGTTATTGTCAAAT-3’ |
rcagIXhoI | 5’-ACCGCTCGAGTAAAAAACATTTCACATCT-3’ |
fcag8BNotI | 5’-GGTGGCGGCCGCCAATGAGTGTATTATTTC-3’ |
rcag8BKpnI | 5’-GCCCGGTACCATAATAAAGCAACGGATC-3’ |
Alphabets ‘f’ and ‘r’ indicate forward and reverse primers used to construct plasmids.