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. 2013 Sep 10;8(9):e74244. doi: 10.1371/journal.pone.0074244

Figure 2. PrPC in DRM preparation of young (3-4 months) vs. old (20-21 months) mice.

Figure 2

Western blot analysis of DRMs prepared from equal amounts of total hippocampal protein extracts (100 µg of total protein) at the indicated ages. Young = 3-4 months old; old = 21-22 months old. Each lane corresponds to a single animal. Antibodies used: D18 (1:1,000; InPro Biotechnology, Inc, South San Francisco), mouse monoclonal anti flotillin1 (1:1,000; BD Biosciences). Relative PrPC amounts from 3 mice per time point were analyzed. Each data point represents the relative protein level normalized over flotillin1 ± SD.