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. 2013 Sep 10;8(9):e74244. doi: 10.1371/journal.pone.0074244

Figure 4. PrPC in DRMs from functional synaptosomes treated with sphingomyelin.

Figure 4

Western blot analysis of DRMs prepared from synaptosomes treated with sphingomyelin for 30 min (100 µg/mL) and relative controls. Antibodies used: D18 (1:1,000; InPro Biotechnology, Inc, South San Francisco), mouse monoclonal anti flotillin1 (1:1,000; BD Biosciences), mouse monoclonal anti vinculin (1:5,000; Sigma-Aldrich). Relative PrPC amounts from 3 preparations per condition. Each data point represents the relative PrP level normalized over vinculin ± SD. Sphingomyelin treatment determined about 30% increase in PrPC levels in DRMs.

*: p<0.05.