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Confocal images of fully developed hippocampal primary neurons (21 DIV). Normal (above) and surface immunolabeling (below) of PrPC (green) coupled with synaptophysin and PSD95 (red). Antibodies used: D18 (10 µg/mL and 20 µg/mL in surface immunolabeling InPro Biotechnology, Inc, South San Francisco), mouse monoclonal anti Synaptophisin (1:100; SySy), mouse monoclonal anti PSD95 (1:100; Sigma).
Scale bar = 10 µm.
