Skip to main content
. 2013 Sep 10;8(9):e74385. doi: 10.1371/journal.pone.0074385

Figure 3. sST2 is secreted by granulosa cells.

Figure 3

Aspirated FFs contained a mixture of granulosa cells (CD45 negative) and leukocytes (CD45 positive) (A). Leukocytes were removed by filtration through a 70 µm filter which retained granulosa cells, resulting in >98% purity granulosa cells (B). Full length IL-33 and ST2 in the purified granulosa preparations can be detected by immunoblotting from three patients (1, 2, 3), PL = placenta lysate positive control (C). Purified granulosa secrete sST2 in culture (D), granulosa cell preparations from three patients (red diamonds, green triangles, blue circles) were cultured for up to 72 hours and supernatants assayed by ELISA for sST2. Growth media contained no detectable sST2.