Figure 2. Outline of the Tropaeolum transformation procedure.

Seeds are sown on soil and grown until at least four leaves have developed (app. 2 weeks). Agrobacteria carrying the construct of interest are streaked from glycerol cultures onto LB (incl. Antibiotics) plates for 2 days. Loops of cells are briefly washed and resuspended in infiltration liquid, adjusted to OD600nM 0.2, incubated for 3 hours and subsequently infiltrated into the leaves.