Figure 5. Luciferase activity in transgenic Tropaeolum majus tissue.

T . majus leaves were infiltrated with agrobacteria carrying a construct for constitutive expression of the luciferase gene. 5 days post-infiltration, an area of the leaf (top left) was infiltrated with substrate solution (10 mM MgCl2, 10 mM MES pH 5.7, 0.5% DMSO, 0.1 mM luciferin), followed by chemiluminescence detection. Left: chemiluminescence (Chemdoc Biorad, exposure 7 sec.). Right: brightfield image of leaves, showing the luciferin-infiltrated area. Note that some areas were not infiltrated with substrate solution only, but not with agrobacteria and vice versa to document that the signal is solely due to expression of the reporter transgene.