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. 2013 Sep 10;8(9):e75869. doi: 10.1371/journal.pone.0075869

Figure 5. Overexpression of KCNL-2 in the N2 background causes a Hyperactive Egg-Laying Phenotype.

Figure 5

A. An unlaid egg assay was carried out by dissolving the cuticle of adult worms in 1.8% sodium hypochlorite and shows that the number of eggs in utero in transgenic lines that overexpress KCNL-2 are significantly less than the number of eggs retained in utero in N2 and kcnl-2(tm1885) strains (p<0.001, Student’s t test), while kcnl-2(tm1885) has a significantly increased number of eggs retained in utero relative to N2 worms (p<0.001, Student’s t test). B) Representative images of worms from each strain. C) Egg-staging assays reveal that the proportion of young eggs from KCNL-2(OE-1) (strain VK1000) (54.82±7.65%) and KCNL-2(OE-2) (strain VK1065) (60.57±12.48%) was significantly greater than those of N2 worms (2.78±4.81%) (n=3, p<0.05; Kruskal–Wallis H test).