Skip to main content
. 2013 Sep 10;8(9):e75869. doi: 10.1371/journal.pone.0075869

Figure 6. Rescue of the mild Egl phenotype shown by kcnl-2(tm1885) worms.

Figure 6

A. kcnl-2(tm1885) animals retain a significantly greater number of eggs in utero relative to N2 animals (P<0.0001). The number of eggs in utero in heterozygotes (kcnl-2(-/+)) is significantly greater than that of the N2 animals (p=0.007), but significantly less than the average number of eggs found in kcnl-2(tm1885) animals (p=0.008). B. Transformation of kcnl-2(tm1885) with 1 ng/µl, 10 ng/µl or 90 ng/µl of p kcnl-2 kcnl-2(taa2)::gfp gives the transgenic lines KCNL-2(OE-3) (strain VK2220), KCNL-2(OE-4) (strain VK1004) and KCNL-2(OE-5) (strain VK1041), respectively. The average number of eggs retained in utero by KCNL-2(OE-3) was not significantly different from the N2 animals (P=0.8) but was significantly different from the kcnl-2(tm1885) organisms (p<0.0001). KCNL-2(OE-4) and KCNL-2(OE-5) retain fewer eggs in utero than N2 animals (p<0.005; p<0.0001, respectively) and kcnl-2(tm1885) animals (p<0.0001) (Student’s t test). C. Egg-staging assays show that the proportions of young eggs from KCNL-2(OE-2) (54.0±4.3%), KCNL-2(OE-4) (46.0±3.9%), and KCNL-2(OE-5) (46.6±6.7%) are significantly greater than those of N2 (7.2±1.3%) & kcnl-2(tm1885) worms (1.6±1.6%) (p<0.05; n=3, Kruskal–Wallis H test).