Figure 4. Interaction of the promoter of Peg3/Usp29 with ECRs.

Potential long-range interaction of the Peg3 promoter was tested using the +1 oligonucleotide as a base primer along with a set of oligonucleotides that are derived from multiple regions of the Peg3 domain. The initial survey was performed with a fixed number of cycles (36 cycles) using the three libraries derived from neonatal brain, testis and liver, and these results are shown on bottom. Quantitative PCR analyses were also performed. The results are summarized with graphs on top. For this series of analyses, the Ct (threshold cycle) value for each primer set was first calculated from the control library with ligation, and subsequently used as an internal control for the normalization of the Ct values derived from the three tissue libraries.