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. 2013 Sep 10;8(9):e75417. doi: 10.1371/journal.pone.0075417

Figure 6. Epigenetic modifications and evolutionary conservation of ECR18.

Figure 6

(A) Immunoprecipitated DNA with anti-H3K27ac antibodies was first amplified with PCR, and the subsequent PCR products were digested with an enzyme to differentiate parental alleles for a given locus. The promoter regions of imprinted genes show mono-allelic or one allele-biased patterns whereas ECR18 shows a bi-allelic pattern similar to those seen the input DNA. (B) DNA mthylation analysis on ECR18. The bisulfite-converted DNA from male and female neonates were used for PCR amplification. The amplified PCR products were digested with TaqI, and the digestion by this enzyme indicates methylation on the original DNA. (C) The panel represents a snapshot of UCSC Genome Browser showing the peaks of the two histone modifications, H3K27ac and H3K4me1, detected in the human PEG3 domain.