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. 2013 Sep 10;8(9):e74766. doi: 10.1371/journal.pone.0074766

Figure 1. Generation of homozygous Msh6 mutant ESC lines.

Figure 1

Sequence analysis of (A) Msh6 +/+, Msh6 PR/+ and Msh6 PR/PR genomic DNA, (B) Msh6 +/+, Msh6 RH/+ and Msh6 RH/RH genomic DNA and (C) Msh6 +/+, Msh6 LQ/+ and Msh6 LQ/LQ genomic DNA. Single letter amino acid codes are given below the sequence. (D) Whole cell lysates were analyzed for MSH6 and MSH2. γ-Tubulin was used as a loading control. ‘-‘ indicates a knockout allele. The relative percentages of MSH6 levels are indicated.