Figure 3. Generation of Msh6 ^mut/- heterozygous ESC lines.

(A) Southern blot analysis of the Msh6 ^mut/- and control cell lines, showing loss of one of the Msh6 alleles in Msh6 ^mut/- cells. (B and C) Western blot analysis of mutant Msh6 homozygous and heterozygous cell lines and controls. Whole cell lysates were analyzed for the presence of MSH6 and MSH2. γ-tubulin was used as a loading control. ‘-’ indicates a knockout allele. The relative percentages of MSH6 levels are indicated.