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. 2013 Sep 10;8(9):e75175. doi: 10.1371/journal.pone.0075175

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© 2013 Richard et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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T cells from the spleens of MRL/lpr Fli1 ^+/+ and Fli1 ^+/- 10-12 week-old mice were stimulated with anti-CD3/CD28 for 0, 3 and 24 hours and cell death measured by flow cytometry following Annexin V and PI staining. A) Annexin V+/PI- (apoptosing cells), B) Annexin V+/PI+ (dead cells), and C) Annexin V-/PI- (live cells). The ‘n’ represents data from individual animals.