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. 2013 Sep 10;8(9):e74895. doi: 10.1371/journal.pone.0074895

Figure 1. Knockdown of Orai channels using recombinant adenoviruses expressing shRNA.

Figure 1

(A) Knockdown efficiency of shRNAs against Orai1 (Orai1 v1) and Orai2 (Orai2 v1) was determined in HLMCs 4 days following transduction with virus by quantitative RT-PCR (n=3 independent experiments using HLMCs from 3 donors), * p<0.05 compared to expression in cells transduced with luciferase control. All other conditions were not significantly different to control. (B) Transduction of HLMCs with adenoviruses expressing shRNAs against Orai1 (Orai1 v1) and Orai2 (Orai2 v1) (n=3 donors), had no significant effect on cell viability after 4 days compared to luciferase control virus.