Figure 2. Knockdown of Orai1 but not Orai2 significantly reduces Ca²⁺ influx in activated HLMCs.

Whole cell patch clamp current-voltage (I–V) curves from HLMCs transduced with recombinant adenoviruses expressing (A) shRNA against luciferase (n=19 cells), or (B) against Orai1 (n=13); and (C) shRNA against luciferase (n=11) or against Orai2 n=14 (D). (●) baseline current, (■) current elicited following dialysis of HLMCs with 30 µM IP₃ for 4 min, (○) current following the addition of 1 µM GSK-7975A. Currents were recorded in 2 mM external Ca²⁺ and are shown as mean ± SEM. *p=0.0292 compared with corresponding curve in (B).