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Western blot validation of the labeled proteins. (A) Clusterin (50 kDa) and (B) tenascin XB (80 kDa) levels decreased in AS cultures (P1 to P4) with respect the controls (C1 to C4). In contrast (C) gelsolin (75 kDa), (D) pentraxin 3 (37kDa), and (E) pigment epithelium derived factor (PDEF, 50 kDa) augmented in AS cultures when compared with controls. Corresponding p values (Student′s t test) for each protein analyzed are shown. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.
