Fig. 6.
Quantitative analysis of N-glycans and secreted hyaluronan. (a) Cellular pyridylaminated N-glycans in both cell lines were quantified by HPLC with fluorescence detection. The data are represented as pmol per 1 × 107 cells. The amounts in the six categories (high mannose, asialo, monosialo, disialo, trisialo and tetrasialo types) of N-glycans in Hepa1–6 and Min6 cells were quantified based on the peak areas of the chromatograms. B, Secreted hyaluronic acids were quantified by ELISA, and the data represented as pmol per 1 × 107 cells. The amount of secreted hyaluronic acid in mouse fibroblasts that are known to highly express hyaluronic acid was also quantified as a control sample.