Table II. Additional putative glycoproteins identified by mudPIT.
| Gene | Acc. # | Protein name | Predicted MW (kDa) | Function/sequence features | Subcellular localizationa | Spect- counts | Uni-pep | Seq-cov% |
|---|---|---|---|---|---|---|---|---|
| HP0025 | AAD07106 | omp2 | 81.6 | Outer membrane protein | Outer membrane | 5 | 3 | 7.9 |
| HP0191 | O06914 | frdB | 29.3 | Fumarate reductase beta | Inner membrane | 15 | 4 | 26.2 |
| HP0599 | AAD07662 | hylB | 51.0 | Hemolysin secretion | Outer membrane | 31 | 8 | 29.5 |
| HP0371 | AAD07435 | fabE | 18.1 | Biotin carboxyl carrier | Unknown | 35 | 4 | 26.7 |
| HP0110 | P55970 | grpE | 23.3 | Heat shock protein | Cytoplasm | 14 | 5 | 29.9 |
| HP0631 | AAD07691 | hydA | 44.6 | Quinine reactive hydrogenase | Inner membrane | 4 | 3 | 9.1 |
| HP1198 | O25806 | rpoBC | 339.5 | RNA polymerase | Cytoplasm | 9 | 6 | 3.6 |
| HP1496 | P56078 | ctc | 20.9 | General stress protein | Cytoplasm | 6 | 3 | 33.7 |
| HP0824 | P66928 | trxA | 12.5 | Thioredoxin A | Cytoplasm | 20 | 2 | 26.8 |
| HP0825 | 3ISH_B | trxB | 35.5 | Thioredoxin reductase | Cytoplasm | 22 | 6 | 33.1 |
| HP1458 | AAD08500 | — | 12.4 | Thioredoxin | Cytoplasm | 47 | 2 | 26.4 |
| HP1325 | O25883 | fumC | 53.6 | Fumarase | Cytoplasm | 23 | 5 | 25.5 |
| HP1555 | P55975 | tsf | 41.6 | Translation elongation factor | Cytoplasm | 17 | 3 | 9.1 |
| HP0266 | O25045 | pyrC | 44.6 | Dihydroorotase | Unknown | 19 | 5 | 25.1 |
| HP1135 | AAD08177 | atpH | 21.3 | ATP synthase F1 delta | Membrane | 10 | 2 | 18.0 |
| HP1134 | P55987 | atpA | 58.1 | ATP synthase F1 alpha | Membrane | 28 | 5 | 13.2 |
| HP1132 | P55988 | atpD | 54.1 | ATP synthase F1 beta | Membrane | 26 | 6 | 22.3 |
| HP1099 | AAD08142 | eda | 23.9 | Aldolase | Cytoplasm | 5 | 3 | 23.2 |
| HP0154 | P48285 | eno | 49.1 | Enolase | Cytoplasm | 12 | 4 | 21.6 |
| HP0512 | P94845 | glnA | 57.4 | Glutamate synthase | Cytoplasm | 32 | 4 | 15.4 |
| HP0779 | AAD07828 | acnB | 97.7 | Aconitase | Cytoplasm | 72 | 10 | 19.9 |
| HP0163 | P56074 | hemB | 38.1 | Delta-aminolevulinic acid dehyratase | Cytoplasm | 5 | 3 | 15.2 |
| HP0974 | P56196 | pgm | 57.7 | Phosphoglycerate mutase | Cytoplasm | 5 | 4 | 18.1 |
| HP0294 | O25067 | aimE | 39.7 | Aliphatic amidase | Cytoplasm | 31 | 3 | 17.9 |
| HP0649 | P56149 | aspA | 54.6 | Aspartate ammonia lyase | Cytoplasm | 17 | 5 | 17.5 |
| HP0865 | O25536 | dut | 16.8 | Nucleotidohydrolase | Unknown | 3 | 1 | 17.5 |
| HP0020 | AAD07088 | nspC | 48.2 | Carboxynorpermidine decarboxylase | Unknown | 5 | 4 | 16.9 |
| HP1540 | AAD08580 | fbcF | 19.2 | Ubiquinol cytochrome c oxidase | Inner membrane | 18 | 2 | 15.9 |
| HP0480 | O25225 | yihK | 69.9 | GTP-binding | Inner membrane | 10 | 5 | 14.1 |
| HP0954 | O25608 | — | 25.4 | NAD(P)H reductase | Cytoplasm | 3 | 2 | 14.0 |
| HP0176 | P56109 | tsr | 35.7 | Fructose-bisphosphate aldolase | Cytoplasm | 3 | 2 | 13.8 |
| HP1110 | AAD08154 | porA | 47.0 | Pyruvate ferredoxin oxidoreductase | Unknown | 47 | 4 | 18.9 |
| HP1111 | AAD08155 | pfor | 36.9 | Pyruvate ferredoxin oxidoreductase beta | Unknown | 37 | 3 | 16.9 |
| HP0632 | AAD07692 | hydB | 67.6 | Quinone-reactive Ni-Fe hydrogenase | Inner membrane | 7 | 4 | 13.5 |
| HP0026 | P56062 | gltA | 50.9 | Citrate synthase | Cytoplasm | 71 | 6 | 13.2 |
| HP0757 | AAD07805 | — | 34.9 | Beta-alanine synthase | Cytoplasm | 6 | 2 | 12.4 |
| HP0485 | AAD07551 | — | 37.7 | Catalase-like protein | Periplasm | 4 | 3 | 12.3 |
| HP0201 | AAD07269 | plsX | 38.4 | Fatty acid phospholipid synthesis | Cytoplasm | 5 | 2 | 12.1 |
| HP0183 | P56089 | glyA | 48.0 | Serine hydroxyl-methyltransferase | Cytoplasm | 17 | 3 | 11.9 |
| HP0690 | AAD07742 | fadA | 43.4 | Acetyl coenzyme acetyltransferase | Cytoplasm | 5 | 2 | 11.9 |
| HP0402 | P56145 | pheT | 89.4 | phenylalanyl-tRNA synthase | cytoplasm | 20 | 5 | 11.7 |
| HP0096 | AAD07165 | — | 36.7 | phosphoglycerate dehydrogenase | cytoplasm | 6 | 2 | 11.7 |
| HP0576 | O25300 | lepB | 35.3 | signal peptidase I | inner membrane | 11 | 3 | 11.1 |
| HP1013 | O25657 | dapA | 34.8 | dihydrodipicolinate synthetase | cytoplasm | 8 | 2 | 10.8 |
| HP0397 | AAD07461 | serA | 60.8 | phosphoglycerate dehydrogenase | cytoplasm | 12 | 3 | 10.7 |
| HP1576 | O26096 | abc | 38.6 | ABC transporter | inner membrane | 4 | 2 | 10.7 |
| HP0006 | P56061 | panC | 32.8 | pantoate-beta-alanine ligase | cytoplasm | 2 | 2 | 10.7 |
| HP0558 | NP_207353 | fabF | 45.8 | beta ketoacyl-acyl carrier protein synthase | inner membrane | 8 | 2 | 10.6 |
| HP0330 | O25097 | ilvC | 38.5 | ketol-acid reductoisomerase | cytoplasm | 2 | 2 | 10.3 |
| HP0672 | AAD07733 | aspB | 45.1 | mitochondrial signature protein | cytoplasm | 2 | 2 | 10.0 |
| HP1103 | O25731 | glk | 38.6 | glucokinase | cytoplasm | 11 | 2 | 9.9 |
| HP0221 | AAD07289 | — | 38.3 | nifU-like | cytoplasm | 2 | 2 | 9.9 |
| HP0604 | AAD07669 | hemE | 40.4 | uroporphyrinogen decarboxylase | cytoplasm | 3 | 2 | 9.8 |
| HP0306 | P56115 | hemL | 49.3 | aminomutase | cytoplasm | 3 | 2 | 9.7 |
| HP1266 | AAD08310 | nqo3 | 99.1 | NADH-ubiquinone oxidoreductase | unknown | 8 | 4 | 9.3 |
| HP1366 | AAD08410 | mboIIR | 52.6 | type IIS restriction enzyme R | unknown | 4 | 2 | 8.5 |
| HP0490 | AAD07558 | — | 45.2 | putative potassium channel | unknown | 2 | 2 | 8.5 |
| HP1181 | AAD08227 | mfs | 51.4 | multidrug-efflux transporter | inner membrane | 4 | 2 | 8.4 |
| HP0777 | P56106 | pyrH | 27.4 | uridine 5′ monophosphate kinase | cytoplasm | 13 | 2 | 8.3 |
| HP1422 | P56456 | ileS | 111.3 | Isoleucyl-tRNA synthetase | cytoplasm | 5 | 4 | 8.1 |
| HP0322 | AAD07390 | — | 61.4 | poly E-rich protein | cytoplasm | 5 | 3 | 7.9 |
| HP0382 | AAD07451 | — | 48.5 | zinc metallo protease | unknown | 3 | 2 | 7.9 |
| HP0859 | AAD07905 | rfaD | 39.4 | mannoheptose epimerase | cytoplasm | 5 | 2 | 7.8 |
| HP1100 | P56111 | — | 70.2 | 6-phophogluconate dehydratase | cytoplasm | 55 | 2 | 6.4 |
| HP0210 | P56116 | htpG | 74.8 | chaperone and heat shock protein | cytoplasm | 2 | 2 | 7.2 |
| HP0056 | AAD07126 | — | 141.6 | pyrroline-5-carboxylate dehydrogenase | cytoplasm | 14 | 5 | 7.1 |
| HP0728 | O25428 | tilS | 41.7 | tRNA(Ile)-lysidine synthetase | unknown | 3 | 2 | 7.1 |
| HP1045 | O25686 | acoE | 78.8 | acetyl-CoA synthetase | cytoplasm | 7 | 3 | 6.7 |
| HP0169 | P56113 | prtC | 49.9 | collagenase | unknown | 2 | 2 | 6.7 |
| HP1104 | AAD08150 | cad | 40.6 | cinnamyl-alcohol dehydrogenase | cytoplasm | 7 | 2 | 6.6 |
| HP0470 | AAD07532 | pepF | 70.9 | oligoendopeptidase F | cytoplasm | 5 | 3 | 6.4 |
| HP0422 | AAD07486 | speA | 74.0 | arginine decarboxylase | periplasm | 7 | 3 | 6.3 |
| HP0510 | P94844 | dapB | 29.4 | dihydrodipicolinate reductase | cytoplasm | 4 | 2 | 6.3 |
| HP0680 | P55982 | nrdA | 94.7 | Ribonucleoside-diphosphate reductase | Cytoplasm | 2 | 2 | 5.5 |
| HP1430 | P56185 | — | 81.3 | Conserved hypothetical ATP-binding protein | Unknown | 4 | 2 | 5.5 |
| HP1012 | AAD08056 | pqqE | 52.9 | Protease | Unknown | 2 | 2 | 5.3 |
| HP1213 | O25812 | pnp | 80.8 | Polynucleotide phosphorylase | Cytoplasm | 2 | 2 | 4.8 |
| HP1278 | P56142 | trpB | 45.0 | Tryptophan synthase beta | Cytoplasm | 16 | 1 | 4.6 |
| HP1072 | P55989 | copA | 86.1 | Copper-transporting ATPase | Inner membrane | 3 | 2 | 4.3 |
| HP1450 | O25989 | yidC | 65.8 | Protein translocase | Inner membrane | 6 | 2 | 4.3 |
| HP0121 | P56070 | ppsA | 95.8 | Phosphoenolpyruvate synthase | Cytoplasm | 3 | 2 | 4.1 |
| HP0607 | NP_207402 | acrB | 119.6 | Acriflavine resistance | Inner membrane | 2 | 2 | 4.0 |
| HP1402 | AAD08445 | hsdR | 121.7 | Type I restriction enzyme | Unknown | 2 | 2 | 3.4 |
| HP1112 | P56468 | purB | 52.6 | Adenylosuccinate lyase | Cytoplasm | 5 | 1 | 3.4 |
| HP1478 | AAD08516 | uvrD | 81.7 | DNA helicase II | Cytoplasm | 2 | 2 | 3.3 |
| HP1547 | P56457 | leuS | 97.7 | Leucyl-tRNA synthase | Cytoplasm | 3 | 2 | 2.9 |
| HP0791 | Q59465 | cadA | 78.9 | Cadmium-transporting ATPase | Inner membrane | 2 | 2 | 2.9 |
| HP0600 | AAD07663 | spaB | 71.8 | Multidrug resistance | Inner membrane | 3 | 1 | 2.7 |
| HP0696 | AAD07747 | — | 90.7 | N-methylhydantoinase | Unknown | 3 | 2 | 2.6 |
| HP0958 | — | 31.3 | Hypothetical protein | Unknown | 2 | 2 | 12.3 | |
| HP0787 | — | 48.2 | Conserved integral membrane protein | Inner membrane | 21 | 3 | 9.3 | |
| HP0231 | — | 31.1 | Hypothetical protein | Unknown | 10 | 4 | 22.9 | |
| HP0318 | — | 30.1 | Hypothetical protein | Unknown | 35 | 3 | 18.4 | |
| HP0086 | — | 53.3 | Hypothetical protein | Unknown | 35 | 3 | 9.1 | |
| HP0773 | — | 41.9 | Hypothetical protein | Unknown | 32 | 2 | 8.9 | |
| HP0754 | — | 9.7 | Hypothetical protein | Unknown | 7 | 2 | 34.1 | |
| HP0396 | — | 74.4 | Hypothetical protein | Unknown | 3 | 3 | 8.8 | |
| HP1079 | — | 45.2 | Hypothetical protein | Unknown | 34 | 2 | 6.9 | |
| HP1143 | — | 53.2 | Hypothetical protein | Unknown | 4 | 1 | 5.0 |
a Subcellular localization was based on literature reports or predicted using PSORTb v.3.0.