Table 1. IC50 values for total IgG against different antigens in GIA.
Antigen | End-point Titre | End-point IgG Concentration ng/ml | IC50# 3D7 g/ml | IC50# W2mef g/ml | IC50# FCR3 g/ml | Average IC50 3 strains | |
EBA-175 R3-5 (3D7) | 1/1,280,000 | 16 | 9 | 30 | <16 | 17 | Fig. 2A |
EBA-175 R3-5 (W2mef) | 1/2,560,000 | 8 | 37 | 45 | 60 | 47 | Fig. 2C |
EBA-175 F2-R5 (3D7) | 1/2,560,000 | 8 | 56 | 85 | 50 | 64 | Not shown |
EBA-175 R4-5 (3D7) | 1/2,560,000 | 8 | 198 | 250 | 175 | 208 | Fig. 2H |
EBA-140 R3-5 (3D7) | 1/5,120,000 | 4 | 200 | 1250 | 1250 | 900 | Fig. 4 |
EBA-175 R2 (3D7) | 1/1,280,000 | 16 | NA | NA | NA | NA | |
PfRh2-NTR rbc binding | 1/10,240,000 | 2 | NA | NA | NA | NA | |
PfRh2-2A9 CTR | 1/5,120,000 | 4 | NA | NA | NA | NA |
IC50 values were calculated by interpolation on the plot of total IgG[log10] versus % GIA with measured points connected by straight lines (as described in [56], calculations were performed on % GIA values in a 1-cycle assay, with IgG titrated from 2 mg/ml.