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. 2013 Sep 10;8(9):e72504. doi: 10.1371/journal.pone.0072504

Table 1. IC50 values for total IgG against different antigens in GIA.

Antigen End-point Titre End-point IgG Concentration ng/ml IC50# 3D7 g/ml IC50# W2mef g/ml IC50# FCR3 g/ml Average IC50 3 strains
EBA-175 R3-5 (3D7) 1/1,280,000 16 9 30 <16 17 Fig. 2A
EBA-175 R3-5 (W2mef) 1/2,560,000 8 37 45 60 47 Fig. 2C
EBA-175 F2-R5 (3D7) 1/2,560,000 8 56 85 50 64 Not shown
EBA-175 R4-5 (3D7) 1/2,560,000 8 198 250 175 208 Fig. 2H
EBA-140 R3-5 (3D7) 1/5,120,000 4 200 1250 1250 900 Fig. 4
EBA-175 R2 (3D7) 1/1,280,000 16 NA NA NA NA
PfRh2-NTR rbc binding 1/10,240,000 2 NA NA NA NA
PfRh2-2A9 CTR 1/5,120,000 4 NA NA NA NA
#

IC50 values were calculated by interpolation on the plot of total IgG[log10] versus % GIA with measured points connected by straight lines (as described in [56], calculations were performed on % GIA values in a 1-cycle assay, with IgG titrated from 2 mg/ml.