Figure 2. Expression of wild-type β-TM_EGFP and E41K-β-TM_EGFP in human cells.

(A–B) Wild-type β-TM_EGFP and (C–D) E41K-β-TM_EGFP mutants were transfected in human (A and C) myoblasts and (B and D) myotubes differentiated for three to six days and labeled with TRITC-phalloidin (red) and DAPI (blue) to highlight cell nuclei. (A) WT-β-TM was expressed in human myoblasts or (B) myotubes and incorporated well into endogenous filamentous actin as visualised with phalloidin (long arrows). The E41K-β-TM mutant induced diffuse intranuclear and cytoplasmic clouds in transfected myoblasts (C–Ć ´’’) and perinuclear aggregates in myotubes (D–D´ ´’’; short arrows). Confocal microscopy was performed using a Zeiss LSM 510 Meta confocal microscope or an LSM 700 inverted Axio Observer.Z1 microscope. Scale bar  = 10 µm.