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. 2013 Sep 10;8(9):e73690. doi: 10.1371/journal.pone.0073690

Figure 5. Pattern of ATPα immunoreactivity in flies with reduced CRY expression in photoreceptors (gmr-GAL4>UAS-cry-RNAi) (A) and glia (repo-GAL4>UAS-cry-RNAi) (B) under LD.

Figure 5

The fluorescence index ± SE is shown as a function of time. (A) In gmr-GAL4>UAS-cry-RNAi flies the higher levels of immunoreactivity were observed at ZT1 and ZT13. The immunosignal decreased by 66.3% at ZT4 and by 58.3% at ZT16. Statistically significant differences were observed between ZT1 and ZT4, ZT1 and ZT16, ZT13 and ZT4, ZT13 and ZT16. (B) In repo-GAL4>UAS-cry-RNAi flies higher immunosignal was observed at ZT1 and ZT13 and then decreased by about 30% in ZT4 and ZT16. There were statistically significant differences between ZT1 and ZT4, ZT1 and ZT16, ZT13 and ZT4, ZT13 and ZT16. Parametric ANOVA Tukey's test; p<0.05. The two stars symbols indicate statistically significant differences between the experimental strains and CantonS controls at different time points.