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. 2013 Sep 10;8(9):e74742. doi: 10.1371/journal.pone.0074742

Figure 5. Effect of HCA and ZnPP on the expression of Bcl-2 and caspase-3 in the lung.

Figure 5

(A) Western blot and densitometry analysis of Bcl-2 protein in lung tissue. β-actin served as a loading control for cytoplasmic proteins. A representative blot is shown. (B) Immunohistochemistry for active caspase-3 in the lung (200× magnification). Ischemia-reperfusion (IR) significantly decreased Bcl-2 protein expression and induced caspase-3 activation in the lung tissue. Hypercapnic acidosis (HCA) treatment significantly increased Bcl-2 protein expression and attenuated the signals for active caspase-3. When zinc protoporphyrin IX (ZnPP) was added, the protective effect was partially blocked. Data are expressed as mean ± D. *significantly different from control (P < 0.05); + significantly different from IR-vehicle (P < 0.05); # significantly different from IR+HCA (P < 0.05), using one-way ANOVA with Bonferroni post-test.