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. 2013 Sep 10;8(9):e73985. doi: 10.1371/journal.pone.0073985

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© 2013 wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A549 cells were transfected with equal doses of scrambled ncRNA or pre-miR-203, or equal doses of control siRNA or si-PKCα. Cell apoptosis profiles were analyzed by flow cytometry. The biparametric histogram shows cells in early (bottom right quadrant) and late apoptotic states (upper right quadrant). Viable cells are double negative (bottom left quadrant). The experiment was repeated three times, and a statistical analysis is shown in the right panel (mean ± S.D.; ** p<0.01).