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. 2013 Sep 10;8(9):e74045. doi: 10.1371/journal.pone.0074045

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© 2013 Brennecke et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) FACS analysis showed efficient scavenging of fluorescent Venus-tagged CXCL12 (orange profile), CXCL12 antagonist P2G (green profile) and chimeric CXCL11-CXCL12 (blue profile) in 143B-LacZ-HA-X7 (HA-X7), but not in control 143B-LacZ-EV (EV) cells. Controls in the absence of fluorescent ligands (red profiles). (B) Time-dependent uptake of CXCL12-Venus by 143B-LacZ-HA-X7 (○), assessed by time-laps confocal microscopy was not observed in 143B-LacZ-EV (●) cells. (C) CXCL12 at indicated increasing concentrations provoked minimal loss of HA-CXCR7 at the surface of 143B-LacZ-HA-X7 analyzed by FACS. (D) 143B-LacZ-HA-X7 (HA-X7) cells exhibited enhanced adhesion to interleukin-1β activated HUVEC compared to 143B-LacZ-EV cells. #,Ω,Δ indicate significant differences (p<0.05) between indicated cell lines and conditions. Values represent the mean ± SEM (n=3).