Figure 4. Histology and immunostaining of paraffin sections showed a lower metastatic load in the auriculum cordis and the lung of 143B-LacZ- EV than of 143B-LacZ-HA-X7 cell-injected mice and expression of CXCL12 in both tissues.

(A) Representative H&E staining of sections of indicated tissues collected from 143B-LacZ-EV (EV) and 143B-LacZ-HA-X7 (HA-X7) cell injected mice. (B) X-gal (blue) and HA immunostaining of metastatic lesions and immunohistochemical localization of CXCL12 in large vessels of the heart base (hv) region and in lung vessels (lv) and in peribronchial tissue (pbt) of representative paraffin sections of indicated tissues collected from EV and HA-X7 cell-injected mice. (C) Control staining of indicated tissues in the absence of primary HA antibodies. Size bars: 50 µm.