Table 1. Summary of RRM1 mutations.
The listed RRM1 mutations include those previous constructed (C70S C109V and Y83V; Yao et al 2007). The conservation of the yeast residue relative to the corresponding human residue is indicated (see Figure 4) as well as whether the alteration involved converting the yeast residue to that of the human.The location of the mutation on the RNA binding surface is also indicated (Deo et al 1999).
| Residue/Alteration | Conserved Yeast to Human |
Change to Human Residue |
RNA Binding Surface |
|---|---|---|---|
| Y41A | Y | N | Y |
| D44A | Y | N | Y |
| E46H S48D | N/N | Y/Y | N/N |
| I57K | N | Y | N |
| I61A | N | Y | N |
| S65L | N | Y | Y |
| C70A | Y | N | Y |
| C70S C109V | Y/N | N/Y | Y/Y |
| R71A | Y | N | Y |
| A73N | N | N | N |
| T77R | N | N | N |
| L79K | N | N | Y |
| Y83V | Y | N | Y |
| D88A E90A | N/N | N/N | N/N |
| A91A R93E | N/N | Y/Y | N/N |
| Q98T | N | Y | N |
| T102D P103V | N/N | Y/Y | N/N |
| R110A | Y | N | Y |
| M112A | Y | N | Y |
| S114A | Y | N | Y |