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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Hear Res. 2013 Jun 18;304:41–48. doi: 10.1016/j.heares.2013.06.003

Fig. 6.

Fig. 6

Protein and RNA expression levels in +/dfwi5 brainstem are not affected by generation. (A) PMCA2 was quantified from several Western blots of +/dfwi5 animals and generation-matched controls. Heterozygote expression is represented as a percentage of wild-type for generational groups N3-N5 (open bars, n = 3 blots), N6 (light gray bars, n = 5 blots) and N10 (dark gray bars, n = 6 blots). There is no statistical difference between generations in a two-way ANOVA. Error bars represent SEM. (B) Total Atp2b2 is reduced in heterozygotes (+/dfwi5) compared to wild-type (+/+) mice with no differences in absolute expression level across generational groups in a two-way ANOVA. A total of 11 samples from control mice were split into three generation groups: N3-N5 (open circles, n = 2), N6 (gray squares, n = 2) and N10 (black triangles, n = 7). A total of 20 +/dfwi5 samples were quantified and split into three generation groups: N3-N5 (open circles, n = 2), N6 (gray squares, n = 9) and N10 (black triangles, n = 9).