G. bethesdensis induces oxidative burst activity in normal CD14+ monocytes. (A) Luminol-enhanced chemiluminescence was measured in monocytes exposed to control buffer, G. bethesdensis or E. coli at MOIs of 100 and 10 bacteria per host cell in the presence of autologous serum. Data are mean + SD for 7 donors. (B) Data from (A) are represented as area under the curve (AUC). (C) Luminol-enhanced chemiluminescence was measured in PMN and monocytes, isolated from the same donors (n=6), exposed to control buffer, G. bethesdensis or E. coli at an MOI of 100 bacteria per host cell in the presence of autologous serum. Data are mean + SD. (D) AUC data for PMN and monocytes, from the same donors (n=6), exposed to bacteria at MOIs of 100 (shown in (C)) and 10 bacteria per host cell in the presence of autologous serum.