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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: J Immunol. 2013 Aug 16;191(6):3297–3307. doi: 10.4049/jimmunol.1300200

FIGURE 7.

FIGURE 7

G. bethesdensis persists inside CGD MDM for 1 week. (A) Normal MDM were incubated ± 65 U IFN-γ/ml for 2 days before co-culture with G. bethesdensis for 1 week at the indicated MOI in the presence of autologous serum. Data are presented as mean + SD (n=6 donors). Linear regression analysis was conducted to assess differences in slope of lines (* = p ≤ 0.05) for PBS control versus IFN-γ treatment. (B) MDM were treated as in (A) at an MOI of 0.25 bacteria per host cell. Data are % control input for 6 normal donors and 6 CGD donors. Wilcoxon paired t-test was used to compare PBS control versus IFN-γ treatment (* = p ≤ 0.05). (C) IFN-γ-treated normal and CGD MDM infected for 1 week with G. bethesdensis at an MOI of 1 bacterium per host cell. Black arrows indicate the presence of bacteria.