Table II.
Cytokine profiles of normal and CGD monocytes and MDM infected with G. bethesdensis or E. coli for 24 hours
| Monocytes | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Normal (n = 7) | CGD (n = 4) | P-values | ||||||||
|
| ||||||||||
| Cytokinea | (I) Control | (II) G. bethesdensis | (III) E. coli | (IV) Control | (V) G. bethesdensis | (VI) E. coli | II vs. IIIc | V vs. VIc | II vs. Vd | III vs. VId |
| IL-1β | 103 (52) | 608 (126) | 5129 (2502) | 74 (84) | 1088 (462) | 14244 (3620) | * | 0.13 | ** | * |
| IL-6 | 278 (180) | 5404 (2619) | 13462 (4781) | 2605 (3102) | 26699 (8989) | 31512 (18983) | * | ns | ** | * |
| IL-8 | 15089 (7437) | 22252 (3276) | 27901 (6308) | 9794 (8272) | 20904 (1738) | 27254 (5620) | 0.08 | 0.13 | ns | ns |
| IL-10 | 1 (1) | 38(47) | 127 (94) | 9(13) | 539 (147) | 528 (469) | * | ns | ** | ** |
| IL-17 | 29 (14) | 76 (13) | 111 (12) | 37 (33) | 93 (11) | 134 (13) | * | 0.13 | 0.11 | * |
| G-CSF | 10 (4) | 217 (160) | 662 (360) | 73 (108) | 2310 (895) | 1938 (1495) | * | ns | ** | * |
| IFN-γ | 82 (45) | 437 (119) | 815 (208) | 177 (187) | 714 (145) | 1154 (130) | * | 0.13 | * | ** |
| CCL-2 | 1587 (1390) | 3004 (950) | 1435 (1047) | 2334 (2218) | 2990 (794) | 873 (569) | * | 0.13 | ns | ns |
| CCL-4 | 1016 (601) | 3974 (1582) | 7047 (2495) | 2275 (2216) | 5155 (485) | 7634 (3947) | * | ns | ns | ns |
| TNF-α | 101 (87) | 2613 (2178) | 11868 (5249) | 223 (241) | 6814 (6075) | 39407 (12606) | * | 0.13 | * | ** |
|
| ||||||||||
| MDM | ||||||||||
| Normal (n=5-7) | CGD (n=4-5) | P-values | ||||||||
|
| ||||||||||
| Cytokineb | (I) Control (n=7) | (II) G. bethesdensis (n=7) | (III) E. coli (n=5) | (IV) Control (n=5) | (V) G. bethesdensis (n=5) | (VI) E. coli (n=4) | II vs. IIIc | V vs. VIc | II vs. Vd | III vs. VId |
|
| ||||||||||
| IL-6 | 2 (2) | 84 (95) | 1890 (1904) | 4 (2) | 60 (78) | 2834 (1912) | ** | * | ns | ns |
| IL-8 | 185 (117) | 1885 (1604) | 6667 (4561) | 436 (418) | 3264 (3083) | 11548 (6601) | * | 0.06 | ns | ns |
| IL-10 | 2 (2) | 10 (11) | 214 (182) | 5 (3) | 13 (10) | 940 (747) | * | * | ns | 0.11 |
| G-CSF | 2 (1) | 6 (4) | 81 (48) | 3 (1) | 8 (4) | 184 (137) | ** | * | ns | ns |
| IFN-γ | 4 (5) | 35 (32) | 151 (85) | 20 (15) | 47 (35) | 198 (91) | * | * | ns | ns |
| CCL-2 | 547 (398) | 972 (880) | 1318 (862) | 1979 (1104) | 2865 (1931) | 2602 (1862) | ns | ns | * | ns |
| CCL-4 | 139 (210) | 902 (1077) | 6330 (10096) | 136 (71) | 1111 (1476) | 8350 (6852) | ns | 0.11 | ns | ns |
| TNF-α | 3 (2) | 118 (114) | 5502 (6627) | 5 (4) | 115 (132) | 8274 (6333) | ** | * | ns | ns |
a
Cytokines from 5×105 monocytes/ml infected at an MOI of 1 are represented as mean (SD) (pg/ml).
b
Cytokines from 2.5×105 MDM/ml infected at an MOI of 0.25 are represented as mean (SD) (pg/ml).
c
Statistical comparisons by exact paired nonparametric Wilcoxon signed rank test,* =p ≤ 0.05, ns = not significant.
d
Statistical comparisons by exact unpaired nonparametric Mann-Whitney test, *=p ≤ 0.05, **=p ≤ 0.01, ns = not significant.