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Chromatogram of serial injections of 60 pM peptide standards in series. A wash injection with 20 µl 60:30:10 (v/v/v) isopropanol:acetonitrile:H2O injected onto the column was carried out every 6 injections. The right image shows a zoomed-in view of one injection where 5 µl peptide standard was preconcentrated, rinsed, and separated for detection within 4 minutes.
