FIGURE 1.

KRN T cell transfer leads to T_FH and GC B cell differentiation upon antigen recognition. (A) CD4⁺ cells from KRN/B6 (K) mice were transferred into Cα^−/− BxN or Cα^−/− B6 hosts and splenocytes were analyzed 8 days later. Transferred cells, identified as CD4⁺ TCRβ⁺, were assessed for T_FH differentiation by CXCR5 and PD-1 expression as well as CXCR5 and intracellular Bcl6 expression. Naïve CD4⁺TCRβ⁺ cells in KRN/B6 splenocytes were used as controls. (B) GC B cells were assessed by Fas and GL-7 expression in the CD19⁺ population. Results are representative of three independent experiments.