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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nitric Oxide. 2013 Feb 21;34:37–46. doi: 10.1016/j.niox.2013.02.079

Figure 1.

Figure 1

Qualitative analysis of NOS2 interactome. A549 cells were infected with an adenovirus expressing E377Q-NOS2-Flag. After 32 h, cells were stimulated with TNFα. Cleared lysates were immunoprecipitated o/n with control IgG-agarose or anti-Flag-M2-agarose, bound proteins were eluted with Flag peptide, separated by SDS-PAGE and visualized by silver staining. The NOS2 IP was subsequently digested in solution with trypsin and analyzed by LC-MS/MS. Based on the relative abundance of identified proteins, several of the abundant bands were assigned to NOS2, HSP90, HSP70 and tubulin isoforms (indicated at right).