Fig. 4.

Effects of DXM, IL-6 and AG490 on the JAK2/STAT3 signaling pathway in the cells of 7TD1-DXM and 7TD1-WD-90. Both cells were treated with 170 μM of DXM, 4 μg/ml of IL-6 or 50 μM of AG490 and incubated at 37°C for 6 hours, and then the cells were collected. Cell lysates were prepared and run on a SDS-PAGE following by Western blot analysis. Beta-actin was used as a loading control. The experiments were carried out at least three times.