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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Structure. 2013 Aug 1;21(9):1571–1580. doi: 10.1016/j.str.2013.06.021

Figure 2. Decapping rates for wild-type and mutant Dcp1/Dcp2 are affected by pH.

Figure 2

(A) Representative time courses of the fraction m7GDP released and the corresponding first-order exponential fits to obtain kobs over a range of pH values for wild-type Dcp1/Dcp2 decapping complex.

(B) Plot of log (kmax) versus pH for wild-type decapping complex (green), K135A (yellow) and E153Q (purple). Symbols are mean of at least 3 independent experiments and error bars shown are standard deviation. Wild-type and K135A are fit using the 4-parameter equation used to model the dependence of kmax on pH (Harris et al., 2000). E153Q is fit to a line due to the linear dependence of kmax on pH.