Figure 5. Lung cancer cells with the PTPN11^N58S/+ mutation were more sensitive to #220–324 than those without the PTPN11^N58S/+ mutation.

Human lung cancer cell lines H596 and H661 were cultured in DMEM supplemented with 10% FBS and #220–324 at the indicated concentrations for 72 hrs (A) or the cells were treated with #220–324 at 20 μM for the indicate times (B). DMSO-treated cells were included as negative controls. Cell numbers were determined using the One Solution Cell Proliferation Assay kit. Experiments were repeated three times. Similar results were obtained in each. Data are presented as mean±S.E.M. from one representative experiment.